Speaker
Balázs Fábián
(Max Planck Institute of Biophysics)
Description
Membrane buckling simulations are routinely analysed using Fourier fits to estimate protein intrinsic curvature. We show that such fits introduce systematic artifacts, despite accurate height profiles. Fitting the analytical Helfrich buckle shape yields consistent curvature distributions and intrinsic curvatures. Applying this approach to Martini 3 simulations, we extract the intrinsic curvature preference of the tetraspanin CD63 in agreement with experimental values for homologs.