Speaker
Dr
Karen Palacio-Rodriguez
(Max Planck Institute of Biophysics)
Description
During mitotic exit, the nuclear envelope seals spindle‑microtubule holes in a process mediated via LEM2‑ESCRT. Cryo-electron tomography (Cryo-ET) shows LEM2-ESCRT filament architecture but not the sealing mechanism. We built a digital twin by integrating Cryo‑ET with multiscale molecular dynamics simulations of filaments in a 90 nm membrane hole, capturing conformational changes, showing how LEM2’s disordered domain engages microtubules, and allowing in‑silico tests of mechanical stress.