Speaker
Description
The isolation of circulating tumor cells (CTCs) with high cell viability for further multi-omic analysis and organoid generation is a turning point in modern oncology. In recent years, CTCs and CTC cluster research have completely changed the roadmap of disruptive translational technologies in oncology, mainly because if adequate CTC isolation without cell modification could be achieved in liquid biopsies, this would mean a paradigm shift in clinical and preclinical oncology. In this work, we present a proof of concept of CTCs isolation using Blister Actuated Laser Induced Forward Transfer (BA-LIFT) and demonstrate that the technique is not only valid for isolation and further single cell sequencing for multiomic analysis, but also for CTCs cell culture, which opens the possibility of using them to study tumor biology and generate new patient-derived models.