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The eco-friendly biosynthesis of silver nanoparticles (AgNPs) using plant leaf extract offers a cost-effective and sustainable route to value-added nanomaterials using plant metabolites as both reductants and capping agents. In this study, silver nanoparticles were synthesised using a green method that employed the leaf extract of Houttuynia cordata (Mosondori), an indigenous medicinal plant found in Assam. The phytochemical test reveals the bioactive compounds found in the plant extract are actively participate as both reducing and capping agents, thereby eliminating the need for hazardous chemicals. The formation of AgNPs by green synthesis was confirmed by a characteristic colour change and further validated by using characterisation techniques, UV-Vis spectroscopy, X-ray diffraction (XRD), and energy-dispersive spectroscopy (EDS). The characterised results show their crystalline nature and confirm elemental compositions, and the calculated average particle size of synthesised silver nanoparticles. Phytochemical test further indicated the involvement of bioactive compounds in nanoparticle stabilisation. As the AgNPs show a significant antibacterial activity, thus suggests potential applications in the field of environmental and healthcare sectors. This work demonstrated the cost-effective, biocompatible, and sustainable way of using locally available medicinal plants for the green synthesis of nanomaterials, and supporting the development of eco-friendly nanotechnology and rural biotechnology initiatives in Assam.