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Description
Material surface properties are considered as critical factor for the study of in Vitro cell growth pattern and activities. It provides advantages not only for study of cellular activity but also for biomedical technology such as tissue engineering. Neuron is a typical cell type widely used to examine cell communication, alignment and differentiations. In the present study, a simple pattern of indium tin oxide (ITO) for neuron culture is introduced. Circular inter-digitated design of ITO electrode was fabricated to capture a small amount of cell for cellular observations. In order to enhance cell attachment, the fabricated electrode surface was modified by poly-L-lysine, a type of extracellular matrix. SH-SY5Y cell, a human neuroblastoma cell line, was cultured on the modified surface and the cell growth was observed periodically. Small electrical field was applied to the culture for a period of time then cells were fixed for SEM imaging. It is clearly demonstrated that the amount of differentiated cell increased from 42% to 63.5% after subjected to small electric field. In addition, the possibility of fabricated nanostructure electrode can indicate the potential utilize as a single cellular activities without cell invasion.
Keywords: Neuron, SH-SY5Y, cell differentiation, ITO electrode, electrical effect
