Speaker
Mr
Hamid Moradi
(Dept. of Physics, Carleton University)
Description
The development of a radiosensitivity predictive assay is an attractive goal in radiation oncology. Since there is a high degree of inter-patient variability in the inherent sensitivity or resistance to therapy, it is crucial to have the ability to identify molecular markers that correlate with sensitivity or resistance to radiation treatment. We have applied Raman micro-spectroscopy (RMS) in vitro to discriminate between the ovarian carcinoma cell lines A2780s (parental wild type) and A2780cp (cisplatin cross radio-resistant variant). These two cell lines represent a good model of tumor tissues of similar origin but with different intrinsic chemo- and radio-sensitivities. Moreover, their radiobiological behavior has been extensively studied and their survival curves under different irradiation schemes are known. The Raman spectra collected from individual cells undergo initial preprocessing (background subtraction, normalization and noise reduction) to yield true Raman spectra representative of the cells. The mean of these spectra are analysed with Principal Component Analysis (PCA) followed by Linear Discriminant Analysis (LDA) to yield a strong separation between the cell lines. The objective of this ongoing work is to characterize the spectral differences between the two cell types in order to determine the underlying biochemical basis for this separation. The multivariate classification model constructed using such Raman spectra of ovarian cancer cells could potentially be utilized for early prediction of tumor response.
Authors
Mr
Abrar Ahmad
(Dept. of Physics, Carleton University)
Mr
Dean Shepherdson
(Dept. of Physics, Carleton University)
Mr
Hamid Moradi
(Dept. of Physics, Carleton University)
Dr
Sangeeta Murugkar
(Dept. of Physics, Carleton University)
Co-authors
Dr
Balazs Nyiri
(Dept. of Medical Physics, The Ottawa Hospital Cancer Centre)
Dr
Barbara Vanderhyden
(Dept. of Cellular and Molecular Medicine, University of Ottawa)
Dr
Gosia Niedbala
(Dept. of Medical Physics, The Ottawa Hospital Cancer Centre)
Dr
Libni Eapen
(Dept. of Medical Physics, The Ottawa Hospital Cancer Centre)
Ms
Nhung Vuong
(Dept. of Cellular and Molecular Medicine, University of Ottawa)
