Speaker
Description
Motivation/Background: Optical Coherence Tomography (OCT) signals provide highly sensitive measures of reflective elements in target tissue. The speckle of an OCT signal can capture cellular dynamics using a set of techniques referred to as dynamic OCT, which produces contrasts corresponding to molecular movements. OCT time signals also contain spectral information which have been used in pulse oximetry (1), intravascular lipid mapping (2), and chromophore identification (3). However, it hasn’t been applied to dynamic OCT methods that track changes in molecular movements over time.
Methods: In this study, a potential methodology for applying spectroscopic information for the development of dynamic OCT contrasts was illustrated. After computing the short-time Fourier transform, spectral contributions of OCT speckle were detected by performing cross correlation of the total complex phasor in each voxel with complex phasors in each spectral bin.
Results: Various spectral bands have shown activity in different layers of cornea, indicating different types of cellular activities. By correlating the evolution of component phasors with the total phasor at an OCT voxel, a series of images from a corneal B-scan over time were created, that show the spectral basis of fluctuations in the OCT signal.
Discussion/Conclusion: This method has the potential to create a new set of dynamic OCT contrast that may reveal unique characteristics of scatterers such as particle size and sub-resolution morphology. With this new dimension of OCT signal analysis, the biological mechanics of OCT biopsied tissues may be described in finer detail.
| Keyword-1 | Optical Coherence Tomography |
|---|---|
| Keyword-2 | Dynamic OCT |
| Keyword-3 | Spectral OCT |