Speaker
Description
Many cells in the retina can contain metabolic and visual cycle molecules with intrinsic fluorescent properties. As a result, adaptive optics single- and two‑photon excited fluorescence ophthalmoscopy offer powerful approaches for examining in vivo the health and function of individual retinal cells. By analyzing both the fluctuations in fluorescence intensity and their timing characteristics, these modalities can provide insight into cellular physiology. Differences observed in time‑resolved fluorescence images reflect the diverse fluorescent sources present across various retinal cell types.
In non‑human primates, two‑photon excited fluorescence signals change in response to visual stimulation and show distinct alterations in models of retinal degeneration and during systemic hypoxia. In human participants, single-photon fluorescence enables visualization of cellular mosaics and detection of molecular variations. Furthermore, in preclinical studies, the introduction of extrinsic fluorophores into cells allows these molecules to serve as optical reporters of retinal function.
| Keyword-1 | fluorescence |
|---|---|
| Keyword-2 | fluorescence lifetime |
| Keyword-3 | adaptive optics |