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The potential of catalytic pyrolysis of non-edible biomass derived from microalgae (Chlorella sp.) for bio-oil using commercial and synthesis nano-crystalline HZSM-5 catalyst in a fixed bed reactor was studied in this work. The Chlorella sp. biomass was used as the feedstock and the chemical and elemental components of the Chlorella sp. were identified by proximate and ultimate analysis. The maximum yield of bio-oil was optimized in order to find the optimum condition of the operation variables. In addition the obtained bio-oils were analyzed by elemental GC–MS and FTIR. The results revealed that at 500 degree C with 2.5 gram of nano HZSM-5 in fixed bed reactor for 90 minutes is the optimum conditions which provides the maximum yield of bio-oil around 33% by weight. The whole bio-oil derived from microalgae biomass is comprised of a various chemical compound of oxygenated compounds, various aromatics and their derivatives, long-chain alkanes, and nitrogenous compounds.
