Speaker
Description
In recent years, treatment approach of malignant neoplasm (cancer), which has high death ratio , has been actively studied. There are several current cancer therapy as radiation therapy, surgical therapy, and chemotherapy (anticancer drugs), but these therapy have some disadvantages. The purpose of this study was to establish a new cancer therapy, using nanosecond pulsed electric fields (nsPEFs), with the less disadvantages.
In vivo and ex vivo experiments using embryonic chick assay and in vitro experiment have been conducted to pulsed electric fields application experiments with 2ns-PEFs for mouse breast cancer cells: EMT6. The pulse-application experiments were carried out with mouse melanoma cells: B16-F10. When 2ns-PEFs were applied on solid tumors and cell suspension in an electroporation cuvette, significant effects were not observed. The cancer cells surviving fraction in suspension measured with WST-1 assay was not different to that of controls. Therefore, a pulse-application experiment with 14ns-PEFs was performed in vitro experiment using cell suspension, and compared with 2ns-PEFs. In this experiment, he cancer cells surviving fraction was measured with WST-1 assay and crystal violet assay. When the 2ns-PEFs were applied, the cancer cells surviving fraction did not changed regardless of the number of pulses. On the other hand, the cancer cells surviving fraction decreased as the number of pulses increased in comparison with controls, when 14ns-PEFs were applied. The effect of pulse width and difference of effect between B16-F10 cells and EMT6 should be considered circumstantially.
