Speaker
Dr
Ahmad Hosseinizadeh
(Univ. of Wisconsin, Milwaukee)
Description
In the absence of extraneous and stochastic data artifacts, differences between X-ray diffraction snapshots of a biomolecule arise from changes in object orientation and conformation. Using the X-ray Free Electron Laser data from the PR772 virus, collected at SLAC National Laboratory, we show that a manifold embedding technique can extract the structure and map the conformational spectrum of this virus. The ability to determine and sort conformational heterogeneity is thus essential for a reliable determination of the three-dimensional structure in single-particle experiments.
Authors
Dr
Ahmad Hosseinizadeh
(Univ. of Wisconsin, Milwaukee)
Dr
Peter Schwander
(UWM)
Dr
Ghoncheh Mashayekhi
(UWM)
Dr
Jeremy Cooperman
(UWM)
Prof.
Abbas Ourmazd
(UWM)
Dr
Ali Dashti
(UWM)
Dr
Russell Fung
(UWM)
Prof.
Marius Schmidt
(UWM)
Prof.
Brenda Hogue
(Arizona State University)
Dr
Andy Aquila
(SLAC)
Dr
Chuck Yoon
(SLAC)
Prof.
Garth Williams
(BNL)
